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AN ACTIN‐LIKE PROTEIN OF THE SEA URCHIN EGGS II. DIRECT ISOLATION PROCEDURE
Author(s) -
MIKINOUMURA TAIKO
Publication year - 1969
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1969.00219.x
Subject(s) - sea urchin , actin , fractionation , chemistry , ammonium sulfate , protein purification , chromatography , isolation (microbiology) , biochemistry , extraction (chemistry) , biology , microbiology and biotechnology
A bstract A direct extraction procedure for isolation of an actin‐like protein from fresh sea urchin eggs was tried. The actin‐like protein was found in the 10,000g supernatant of the egg homogenate. The crude extract obtained from the egg homogenate was purified by ammonium sulfate fractionation and dialysis against an ATP‐cysteine solution. The purified actin‐like protein occurred as 2.8S globular protein units, which transformed into heavy components of 7–8S and 12–13S on addition of salts. This change was accompanied by a rise in viscosity.