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STUDIES ON THE EGG‐MEMBRANE LYSIN OF TEGULA PFEIFFERI : QUANTITATIVE ASSAY OF THE LYTIC ACTIVITY
Author(s) -
HAINO K.,
KIGAWA M.
Publication year - 1969
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1969.00203.x
Subject(s) - lysin , lytic cycle , lysis , membrane , chemistry , chromatography , biology , biophysics , biochemistry , virology , virus , escherichia coli , bacteriophage , gene
A bstract An egg‐membrane lysin was partially purified from the sperm extract of Tegula pfeifferi . A turbidimetric and a chemical method with the use of isolated egg membrane as the substrate have been proposed for the quantitative assay of the lysin. The methods are shown to be valid over a limited range of lysin concentration. The pH optimum for the lytic activity is at about 8.3. The lysin shows considerable thermo‐instability and is highly sensitive to PCMB and heavy metals. The reduction in turbidity of egg‐membrane suspensions during lysis is accompanied by the release of a substance(s) containing neutral sugar. The possibility that the lytic process is composed of two steps is discussed.