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Effects of the angiotensin II receptor blocker losartan on the monocyte expression of biglycan in hypertensive patients
Author(s) -
Sardo Maria A,
Mandraffino Giuseppe,
Riggio Stefania,
D’Ascola Angela,
Alibrandi Angela,
Saitta Carlo,
Imbalzano Egidio,
Castaldo Maria,
Cinquegrani Maurizio,
Saitta Antonino
Publication year - 2010
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.2010.05407.x
Subject(s) - losartan , biglycan , medicine , endocrinology , angiotensin ii , monocyte , peripheral blood mononuclear cell , angiotensin receptor , valsartan , blood pressure , angiotensin ii receptor type 1 , receptor , chemistry , decorin , in vitro , biochemistry , cartilage , proteoglycan , anatomy
Summary 1. Recently, we demonstrated that biglycan (BGN) is increased in circulating monocyte cells from hypertensive patients and that angiotensin (Ang) II is able to increase BGN expression. The present study was designed to investigate the effects of treatment with the angiotensin AT 1 receptor antagonist losartan on monocyte BGN mRNA and protein expression in essential hypertension. 2. One hundred and twenty‐six newly diagnosed hypertensive patients without additional risk factors for atherosclerosis and cardiovascular disease were treated with 100 mg losartan once daily for 6 months. Biglycan mRNA and protein expression was determined in monocytes isolated from peripheral blood before (T 0 ) and after (T 1 ) therapy. Plasma levels of interleukin (IL)‐6, tumour necrosis factor (TNF)‐α and high sensitivity C‐reactive protein (hs‐CRP) were also determined. In addition, BGN mRNA and protein expression was determined after the ex vivo addition of 1 μmol/L AngII to monocytes isolated from 20 randomly selected hypertensive patients. 3. Biglycan mRNA and protein expression, blood pressure and plasma levels of fibrinogen, IL‐6, TNF‐α and CRP were significantly lower at T 1 than at T 0 . Variations in BGN expression were associated with inflammatory markers, but not directly with blood pressure. In AngII‐stimulated monocytes, BGN mRNA and protein expression was significantly lower at T 1 that at T 0 . Moreover, mean BGN mRNA expression in AngII‐stimulated monocytes isolated from losartan‐treated patients was similar to baseline expression in unstimulated monocytes from untreated patients. 4. The results of the present study show that losartan can reduce BGN expression in monocytes from hypertensive patients, without any linear association with blood pressure, suggesting that the effects of AngII on BGN expression in monocytes may be modulated, in part, by an AT 1 receptor blocker.