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Involvement of volume‐sensitive Cl − channels in the proliferation of human subcutaneous pre‐adipocytes
Author(s) -
Hu Hao,
Li DongLing,
Fan Lin,
Ren Jun,
Wang ShengPeng,
Jia Bing,
Liu BingHang,
Sun Lei,
Yu XiaoJiang,
Zang WeiJin
Publication year - 2010
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.2009.05223.x
Subject(s) - chemistry , medicine , endocrinology , volume (thermodynamics) , biophysics , biology , physics , thermodynamics
Summary1 Obesity is a significant challenge in terms of public health and preventive medicine. Inhibition of pre‐adipocyte proliferation is believed to be important in the proposed anti‐obesity mechanism. The aim of the present study was to examine the interplay between Cl − channels and their possible involvement in the proliferation of undifferentiated human pre‐adipocytes. 2 Pre‐adipocytes were isolated from human abdominal subcutaneous adipose tissue. Membrane ion currents were recorded using the whole‐cell patch‐clamp technique. Expression of the Cl − channel ClC‐3 gene and protein was determined by reverse transcription–polymerase chain reaction (RT‐PCR) and western blot, respectively. Cell proliferation was evaluated using the [ 3 H]‐thymidine incorporation assay. 3 Electrophysiological recordings revealed a volume‐sensitive Cl − current ( I Cl.vol ) expressed in pre‐adipocytes that was activated under hyposmotic conditions (external osmolarity decreased by 80%) and inhibited by the Cl − channel blocker tamoxifen. 4 Expression of the ClC‐3 channel gene and protein was confirmed by RT‐PCR and western blot analysis. Blocking I Cl.vol with tamoxifen supressed the proliferation of pre‐adipocytes in a concentration‐dependent manner. 5 Collectively, the results of the present study indicate that the volume‐sensitive Cl − channel participates in regulation of the proliferation of human subcutaneous pre‐adipocytes.