Premium
Placental oxidative stress in malnourished rats and changes in kidney proximal tubule sodium ATPases in offspring
Author(s) -
VieiraFilho Leucio D,
Lara Lucienne S,
Silva Paulo A,
Luzardo Ricardo,
EinickerLamas Marcelo,
Cardoso Henriqueta D,
Paixão Ana DO,
Vieyra Adalberto
Publication year - 2009
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.2009.05212.x
Subject(s) - endocrinology , medicine , offspring , oxidative stress , atpase , angiotensin ii , kidney , chemistry , sodium , ouabain , fetus , pregnancy , biology , enzyme , biochemistry , blood pressure , organic chemistry , genetics
Summary1 Intrauterine malnutrition has been linked to the development of adult cardiovascular and renal diseases, which are related to altered Na + balance. Here we investigated whether maternal malnutrition increases placental oxidative stress with subsequent impact on renal ATP‐dependent Na + transporters in the offspring. 2 Maternal malnutrition was induced in rats during pregnancy by using a basic regional diet available in north‐eastern Brazil. Placental oxidative stress was evaluated by measuring thiobarbituric acid‐reactive substances, which were 35–40% higher in malnourished dams (MalN). Na + pumps were evaluated in control and prenatally malnourished rats (at 25 and 90 days of age). 3 Identical Na + /K + ‐ATPase activity was found in both groups at 25 days (approximately 150 nmol P i /mg per min). However, although Na + /K + ‐ATPase increased by 40% with growth in control rats, it remained constant in pups from MalN. 4 In juvenile rats, the activity of the ouabain‐insensitive Na + ‐ATPase was higher in MalN than in controls (70 vs 25 nmol P i /mg per min). Nevertheless, activity did not increase with kidney and body growth: at 90 days, it was 50% lower in MalN than in controls. The maximal stimulation of the Na + ‐ATPase by angiotensin (Ang) II was 35% lower in MalN than in control rats and was attained only with a much higher concentration of the peptide (10 −10 mol/L) than in controls (10 −14 mol/L). 5 Protein kinase C activity, which mediates the effects of AngII on Na + ‐ATPase was only one‐third of normal values in the MalN group. 6 These results indicate that placental oxidative stress may contribute to fetal undernutrition, which leads to later disturbances in Na + pumps from proximal tubule cells.