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CELLULAR EXPRESSION OF RENAL, CARDIAC AND PULMONARY INDUCIBLE NITRIC OXIDE SYNTHASE IN DOUBLE‐TRANSGENIC MICE EXPRESSING HUMAN RENIN AND ANGIOTENSINOGEN GENES
Author(s) -
Radi Zaher A,
Murad Yahya
Publication year - 2009
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.2008.05120.x
Subject(s) - nitric oxide synthase , kidney , endocrinology , renin–angiotensin system , medicine , pulmonary hypertension , biology , immunohistochemistry , lung , renal medulla , nitric oxide , genetically modified mouse , transgene , pathology , blood pressure , gene , biochemistry
SUMMARY1 Hypertensive mice expressing the human renin (REN) and angiotensinogen (AGT) genes are used as a model for human hypertension. 2 The aim of the present study was to investigate the cellular expression and distribution of inducible nitric oxide synthase (iNOS) using immunohistochemistry in lung, heart and kidney tissues from a model of human hypertension using male and female double‐transgenic (h‐Ang 204/1h‐Ren6) mice and wild‐type C57/BI6J mice as controls. 3 In the kidney, the pattern of iNOS expression in various renal microanatomical regions during hypertension was similar to that of age‐matched controls, except in the medullary ascending limb (MAL). In hypertension, iNOS expression was downregulated in the MAL. No significant differences in iNOS expression were seen between control or hypertensive mice in various cardiac microanatomical locations. In the lungs of hypertensive mice, iNOS expression was upregulated in bronchial airway epithelium and bronchial and vascular smooth muscle cells, but downregulated in alveolar macrophages, alveolar septa and pulmonary vascular endothelial cells. Expression of iNOS was similar between male and female mice in the kidney, heart and lungs. 4 In conclusion, iNOS regulation in hypertension is complex and depends on the cell type in which it is expressed and the localization of the cell type in the cardiorenal and pulmonary systems.