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EFFECTS OF SULPHITE SUPPLEMENTATION ON VASCULAR RESPONSIVENESS IN SULPHITE OXIDASE‐DEFICIENT RATS
Author(s) -
Nacitarhan Cahit,
Kucukatay Vural,
Sadan Gulay,
Ozturk Oktay Hasan,
Agar Aysel
Publication year - 2008
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.2007.04825.x
Subject(s) - chemistry , sodium nitroprusside , acetylcholine , medicine , endocrinology , sodium , superoxide dismutase , histamine , sodium nitrite , sodium tungstate , molybdenum , sulfite oxidase , nitric oxide , aorta , biochemistry , oxidative stress , food science , inorganic chemistry , tungsten , organic chemistry
SUMMARY1 The aim of the present study was to explore the effect of dietary sulphite supplementation on vascular responsiveness in sulphite oxidase (SO)‐deficient rats. 2 Male albino rats were divided into four groups, namely control ( n = 8), sulphite‐treated ( n = 8), SO‐deficient ( n = 8) and sulphite‐treated SO‐deficient ( n = 8) groups. Sulphite oxidase deficiency was induced by administration of a low‐molybdenum diet with concurrent addition of 200 p.p.m. tungsten in the form of sodium tungstate in the drinking water for 9 weeks. Sulphite, in the form of sodium metabisulphite (Na 2 O 5 S 2 ; 25 mg/kg) was given in the drinking water to sulphite‐treated and sulphite‐treated SO‐deficient groups for the last 6 weeks. The vascular responsiveness of isolated aortic rings to acetylcholine (ACh), sodium nitroprusside (SNP) and histamine was investigated in organ baths. 3 The responsiveness of aortic rings to SNP and histamine did not differ significantly between groups. Conversely, there was a significant decrease in ACh‐induced relaxation in aortic rings from the sulphite‐treated SO‐deficient group compared with the control group (pD 2 6.2 ± 0.3 and 7.5 ± 0.1, respectively; P < 0.05). Incubation of aortic rings in the presence of either l ‐arginine or superoxide dismutase significantly improved the ACh‐induced vasorelaxation in sulphite‐treated SO‐deficient group (pD 2 7.2 ± 0.3 and 7.4 ± 0.3, respectively). 4 The findings of the present study suggest that the increased production of reactive oxygen species and the resultant increment in l ‐arginine/nitric oxideconsumption may play a role in the reduced endothelium‐dependent vasorelaxation in sulphite‐treated SO‐deficient rats.