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HYDROGEN SULPHIDE‐INDUCED HYPOTHERMIA ATTENUATES STRESS‐RELATED ULCERATION IN RATS
Author(s) -
Lou LiXia,
Geng Bin,
Du JunBao,
Tang ChaoShu
Publication year - 2008
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.2007.04812.x
Subject(s) - hypothermia , hydrogen sulphide , medicine , anesthesia , chemistry , pharmacology , organic chemistry , sulfur
SUMMARY1 Hydrogen sulphide (H 2 S) acts as a gaseous cellular messenger and has recently been reported to induce a suspended animation‐like state in mice. The aim of the present study was to investigate the protective role of H 2 S exposure in stress gastric ulcer. 2 In the present study, we used a rat model of water immersion and restraint stress (WRS) to induce the typical stress disease, namely stress gastric ulcer. Rats were treated with WRS for 4 h, with or without pre‐exposure to H 2 S (160 p.p.m. H 2 S for 2.5 h). 3 In H 2 S‐exposed rats, body temperature was significantly reduced by 2.5C ( P < 0.01) and oxygen consumption was reduced by 37.1% ( P < 0.01) compared with control rats. Plasma levels of H 2 S were increased by 20.8% ( P < 0.01) following pre‐exposure. Pre‐exposure to H 2 S significantly reduced the gastric ulcer index, from 24 ± 9 to 9 ± 2 ( P < 0.01), in WRS rats. In addition, WRS increased plasma levels of adrenocorticotropin (ACTH) and corticosterone 4.7‐ and 4.8‐fold, respectively (both P < 0.01). Pre‐exposure to H 2 S markedly suppressed plasma ACTH and corticosterone level by 34.4 and 53.2%, respectively (both P < 0.01), and reduced WRS‐elevated myeloperoxidase (MPO) activity by 19%. In the present study, WRS increased gastric malondialdehyde and conjugated diene content by 42 and 68%, respectively (both P < 0.01), and H 2 S exposure reduced lipid peroxide production. Finally, H 2 S exposure inhibited the WRS‐elevated expression of glucose‐regulated protein 78 and caspase 12, markers of endoplasmic reticulum stress. 4 In conclusion, a low concentration of H 2 S may be a new pharmacological tool for induced hypothermia to prevent severe stress‐induced diseases and multifarious trauma in the clinical setting.