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ANTIPROLIFERATIVE ACTIVITY OF PAEONIFLORIN IS THROUGH CELL CYCLE ARREST AND THE FAS/FAS LIGAND‐MEDIATED APOPTOTIC PATHWAY IN HUMAN NON‐SMALL CELL LUNG CANCER A549 CELLS
Author(s) -
Hung JenYu,
Yang ChihJen,
Tsai YingMing,
Huang HurngWern,
Huang MingShyan
Publication year - 2008
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.2007.04795.x
Subject(s) - paeoniflorin , fas ligand , apoptosis , a549 cell , chemistry , cell cycle checkpoint , cell cycle , fas receptor , ligand (biochemistry) , microbiology and biotechnology , cancer research , receptor , biology , programmed cell death , biochemistry , high performance liquid chromatography , chromatography
SUMMARY1 Paeoniflorin (PF), isolated from the paeony root, is reported to have immunoregulatory, neuromuscular blocking, anticonvulsant, antihyperglycaemic and antihypotensive effects. 2 The present study investigated the antiproliferative activity of PF. The results showed that PF inhibited the proliferation of A549 by blocking cell cycle progression in the G 0 /G 1 phase and inducing apoptosis. 3 An ELISA showed that G 0 /G 1 phase arrest may be due to p53‐independent induction of p21/wild‐type p53‐activated fragment 1 (WAF1). Increased protein expression of Fas/apoptosis‐1 (APO‐1) and its two ligands, membrane‐bound Fas ligand and soluble Fas ligand, may be responsible for the PF‐induced apoptosis. 4 This is the first study to show that the induction of p21/WAF1 and the activity of the Fas/Fas ligand apoptotic system may participate in the antiproliferative activity of PF in A549 cells.