Premium
OLEIC ACID INHIBITS C‐REACTIVE PROTEIN‐INDUCED EXPRESSION OF INDUCIBLE NITRIC OXIDE SYNTHASE IN RAT AORTIC SMOOTH MUSCLE CELLS
Author(s) -
Xu JinWen,
Ikeda Katsumi,
Ikami Takao,
Yamori Yukio
Publication year - 2007
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.2007.04780.x
Subject(s) - nitric oxide synthase , nitric oxide , acetylation , vascular smooth muscle , biochemistry , chemistry , oleic acid , phosphorylation , histone , gene expression , microbiology and biotechnology , biology , enzyme , smooth muscle , gene , endocrinology
SUMMARY1 Oleic acid is the predominant component of olive, sunflower and avocado oil. Many studies have demonstrated that oleate exerts cardiovascular protective effects; however, the mechanism by which it acts remains unclear. 2 In the present study, we examined the hypothesis that oleate can accelerate the C‐reactive protein (CRP)‐induced expression of inducible nitric oxide synthase (iNOS) in vascular smooth muscle cells. 3 Our data show that pretreatment of the cultured vascular smooth muscles cells with oleate for 3 days effectively weakened iNOS expression and nitric oxide production. Incubation with oleate also inhibited nuclear translocation and acetylation of nuclear factor‐κB p65 and reduced acetylation at Lys9 and phosphorylation at Ser10 of histone H3, which is a major regulatory mechanism for chromatin remodelling and gene expression. 4 These findings suggest that oleate has an anti‐inflammatory effect.