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DEVELOPMENT OF AN IN VIVO BIOASSAY METHOD FOR PRIMARY PREVENTIVE SUBSTANCES OF THE METABOLIC SYNDROME – EFFECT OF KINGINKA‐CHA
Author(s) -
Oku Hisae,
Ogawa Yuko,
Iwaoka Emiko,
Ishiguro Kyoko
Publication year - 2007
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.2007.04772.x
Subject(s) - loganin , metabolic syndrome , in vivo , luteolin , chemistry , bioassay , pharmacology , primary metabolite , chlorogenic acid , chromatography , medicine , traditional medicine , high performance liquid chromatography , biochemistry , metabolite , flavonoid , biology , endocrinology , microbiology and biotechnology , diabetes mellitus , genetics , antioxidant
SUMMARY1 We discovered a phenomenon in which the blood flow of vein microcirculation markedly decreased in the pathology of metabolic syndrome using a model of SHR/NDmcr‐cp/cp (SHR/cp) rats. We tried to develop an in vivo assay method to search for substances for primary prevention of the metabolic syndrome using this blood flow decrease as a guide. 2 Using the assay method, we examined various teas that were expected to prevent the metabolic syndrome when consumed daily. We also found that Kinginka‐cha (the buds of Lonicera japonica ) utilized as a folk tea for the improvement of blood flow could be used to prevent metabolic syndrome. HPLC analysis showed that Kinginka‐cha contained few catechins, which are known to be the major active components in black and green teas, thus suggesting the presence of other active compounds. Chlorogenic acid, luteolin, luteolin 7‐glucoside, loganin, sweroside and secoxyloganin were isolated from Kinginka‐cha.