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CHRONIC SUPPLEMENTATION OF CREATINE AND VITAMINS C AND E INCREASES SURVIVAL AND IMPROVES BIOCHEMICAL PARAMETERS AFTER DOXORUBICIN TREATMENT IN RATS
Author(s) -
Santos Ronaldo VT,
Batista Miguel L,
Caperuto Érico C,
Costa Rosa Luis FBP
Publication year - 2007
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.2007.04717.x
Subject(s) - doxorubicin , creatine , creatinine , lactate dehydrogenase , vitamin e , medicine , endocrinology , creatine kinase , vitamin , ascorbic acid , vitamin c , chemistry , chemotherapy , biochemistry , antioxidant , enzyme , food science
SUMMARY1 Doxorubicin is an anti‐cancer drug with well‐described effects against a wide range of tumours. However, doxorubicin also exhibits dose‐dependent cytotoxicity. The purpose of the present study was to determine whether chronic supplementation of creatine or a mix of vitamins C and E could increase survival and improve plasma parameters 48 h after doxorubicin treatment. 2 Rats were divided into four groups: (i) saline (control); (ii) doxorubicin treated; (iii) a creatine (0.2 g/kg per day)‐supplemented group; and (iv) a vitamin C (250 mg/kg per day) and E (400 IU/kg per day)‐supplemented group. After 30 days supplementation of rats with either creatine or the vitamins, one dose of doxorubicin (15 mg/kg, i.p.) was administered. 3 There was no difference in weight loss among the groups until the 3rd day after doxorubicin treatment, but the creatine‐ and vitamin‐supplemented groups lived longer compared with the doxorubicin only treated group (6, 7 and 3 days, respectively). The doxorubicin‐treated group lost 13.4% bodyweight over 3 days, whereas the creatine‐ and vitamin‐supplemented groups lost approximately 35% 3 days after the administration of doxorubicin. Doxorubicin treatment resulted in an increase in alanine aminotransferase (ALT; P < 0.05), lactate dehydrogenase (LDH; P < 0.05), urea ( P < 0.05) and creatinine ( P < 0.05) compared with levels observed in the control group. Conversely, creatine supplementation promoted a partial return to control values for LDH ( P < 0.05) and creatinine ( P < 0.05), whereas the vitamin mix reversed the changes in ALT ( P < 0.05), LDH ( P < 0.05), urea ( P < 0.05) and creatinine ( P < 0.05). 4 In conclusion, the results of the present study indicate that the two supplementation protocols decreased the cytotoxic effects of doxorubicin and that a protective effect was more noticeable in animals supplemented with the mixture of vitamins C and E.