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EFFECTS OF RESVERATROL ON NUMBER AND ACTIVITY OF ENDOTHELIAL PROGENITOR CELLS FROM HUMAN PERIPHERAL BLOOD
Author(s) -
Wang XiaoBin,
Huang Jun,
Zou JianGang,
Su EnBen,
Shan QiJun,
Yang ZhiJian,
Cao KeJiang
Publication year - 2007
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.2007.04667.x
Subject(s) - resveratrol , vasculogenesis , progenitor cell , endothelial progenitor cell , angiogenesis , endothelial stem cell , in vitro , cell growth , biology , chemistry , pharmacology , microbiology and biotechnology , stem cell , biochemistry , cancer research
SUMMARY1 It has been well established that oestrogens can increase the number of endothelial progenitor cells (EPC) by anti‐apoptotic effects. Resveratrol, a polyphenolic phytoalexin extracted from grapes and wine, has been reported to act as an oestrogen receptor agonist. We hypothesize that putative phyto‐oestrogen may promote EPC proliferation and survival in vitro . 2 Endothelial progenitor cells were isolated from human peripheral blood and identified immunocytochemically. Endothelial progenitor cells were incubated with resveratrol (1, 10, 25 and 50 mmol/L) or control for specified times. Cell proliferation, migration and in vitro vasculogenesis were assayed using the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetra‐zolium bromide (MTT) assay, modified Boyden chamber assay and in vitro vasculogenesis detection, respectively. 3 Resveratrol increased the number of EPC and promoted EPC proliferation, adhesion and migration in a dose‐ and time‐dependent manner. Cell number peaked at 50 mmol/L resveratrol after incubation for 24 h compared with vehicle control (61.3 ± 5.8 vs 112.8 ± 7.2, respectively; P < 0.01). 4 Furthermore, cell cycle analysis showed that 50 mmol/L resveratrol significantly increased the S phase and decreased the G 0 /G 1 phase of EPC. In addition, resveratrol increased vascular endothelial growth factor production and further induced vasculogenesis in vitro . 5 In conclusion, resveratrol significantly induces EPC proliferation, migration and further promotes angiogenesis in vitro .