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IDENTIFICATION OF ANTIMICROBIAL ACTIVITY FROM GOAT CERVICAL EPITHELIUM
Author(s) -
Rana Manish,
Mittal Deepak,
Pereira Ben MJ
Publication year - 2007
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.2007.04646.x
Subject(s) - bacillus subtilis , peptide , antimicrobial , bacteria , antimicrobial peptides , escherichia coli , microbiology and biotechnology , biology , gram positive bacteria , semen , minimum inhibitory concentration , chemistry , biochemistry , anatomy , genetics , gene
SUMMARY1 In mammals, antimicrobial peptides are present in epithelial cells of the respiratory, gastrointestinal and urinogenital tracts. In the present study, we report the identification and partial characterization of an antimicrobial peptide in extracts prepared from goat cervical epithelial tissue. 2 The bioactivity, as determined by radial and disc diffusion assays, was identified in a peptide of molecular mass 4.5 kDa purified by cation exchange and gel filtration techniques. The purified peptide exhibited antibacterial activity against Escherichia coli (Gram‐negative) as well as Bacillus subtilis (Gram‐positive) bacteria. 3 The minimum inhibitory concentration of the peptide estimated by liquid broth assay for E. coli and B. subtilis suspensions was 31 and 50 µg/mL, respectively, a result implying that the peptide is more efficient in inhibiting Gram‐negative than Gram‐positive bacteria. 4 Because the peptide has no spermicidal activity, we propose that it is responsible for protecting the female genital tract against bacteria. 5 Whether the peptide has potentials for use in the cryopreservation, storage and transport of semen remains to be determined.