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IDENTIFICATION OF DIFFERENTIALLY EXPRESSED GENES AFTER HEAT SHOCK IN ISOLATED RAT AORTA
Author(s) -
Kim Jee In,
Kim Su Jin,
Jung Sang Won,
Cho Joon Yong,
Chung Eun Jung,
Kim Moonkyu,
Shin HeungMook,
Kim In Kyeom
Publication year - 2007
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.2007.04620.x
Subject(s) - heat shock protein , heat shock , downregulation and upregulation , gene , biology , microbiology and biotechnology , biochemistry
SUMMARY1 In a previous study, we demonstrated that heat shock augments vascular contractility through the stress response. 2 The current study was designed to identify differentially expressed genes after heat shock by using a novel annealing control primer (ACP) system, which was developed recently to identify authentic genes. 3 Rat aortic rings were mounted in organ baths, exposed to 42°C for 45 min and harvested 4 h after the end of heat shock. Total RNA were used for amplification by the reverse transcriptase–polymerase chain reaction (RT‐PCR) with ACP system. Differentially amplified PCR products were sequenced, searched against the GenBank and confirmed by RT‐PCR. 4 Genes for connective tissue growth factor, stress‐inducible protein 1 and heat shock protein 25 were upregulated, whereas a gene for interferon regulatory factor 1 was downregulated. Immunohistochemistry revealed upregulation of the phosphorylated form of Hsp25 in aortic rings after heat shock. 5 These results suggest that phosphorylated Hsp25 plays a pivotal role in the augmentation of vascular contraction after heat shock.