Premium
SPATIAL PULMONARY FLOW DISTRIBUTION IN RABBIT ISOLATED LUNGS IS A POOR REPRESENTATION OF THE SITUATION IN VIVO *
Author(s) -
Hübler M,
Heller AR,
Bleyl JU,
Rössel T,
Stehr SN,
Koch T
Publication year - 2007
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.2007.04557.x
Subject(s) - in vivo , lung , ex vivo , distribution (mathematics) , dorsum , confidence interval , spatial distribution , anatomy , chemistry , pathology , medicine , biology , mathematics , mathematical analysis , microbiology and biotechnology , statistics
SUMMARY1 Isolated lung preparations are established to investigate effects on pulmonary vascular tone and spatial pulmonary flow ( Q̇ rel ) distribution. In the present study, we hypothesized that Q̇ rel distribution in isolated lungs is only poorly correlated with the in vivo situation. 2 Fourteen rabbits were anaesthetized and mechanically ventilated with room air. Animals were held in an upright position for 15 min and Q̇ rel was assessed using fluorescent microspheres ( Q̇ rel ‐ in vivo ). A second injection of microspheres was made after isolation of the lungs ( Q̇ rel ‐ ex vivo ). Lungs were dried, cut into 1 cm 3 cubes and spatial Q̇ rel distributions were analysed. 3 The mean correlation of Q̇ rel‐in vivo and Q̇ rel‐ex vivo was 0.592 ± 0.188 (95% confidence interval 0.493–0.690). The Q̇ rel was redistributed to more ventral (the mean slope of Q̇ rel vs the dorsal–ventral axis changed from −0.289 ± 0.227 to −0.147 ± 0.114; P = 0.03), cranial (mean slope of Q̇ rel vs the caudal–cranial axis changed from −0.386 ± 0.193 to −0.176 ± 0.142; P < 0.001) and central (mean slope of Q̇ rel vs the hilus–peripheral axis changed from 0.436 ± 0.133 to −0.236 ± 0.159; P = 0.003) lung areas. 4 The results obtained from studies investigating Q̇ rel distributions in isolated lung models must be interpreted cautiously because the isolated lung set‐up significantly affects the spatial distribution of pulmonary flow.