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HEPATOPROTECTIVE AND HYPOLIPIDAEMIC EFFECTS OF GLYCOPROTEIN ISOLATED FROM GARDENIA JASMINOIDES ELLIS IN MICE
Author(s) -
Lee SeiJung,
Oh PhilSun,
Lim KyeTaek
Publication year - 2006
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.2006.04466.x
Subject(s) - chemistry , tbars , gardenia jasminoides , biochemistry , thiobarbituric acid , lipid peroxidation , lactate dehydrogenase , nitric oxide , antioxidant , pharmacology , enzyme , biology , medicine , alternative medicine , organic chemistry , pathology
SUMMARY1 The present study was performed to investigate the hepatoprotective and hypolipidaemic effects of a 27 kDa glycoprotein isolated from Gardenia jasminoides Ellis (GJE glycoprotein) in glucose/glucose oxidase (G/GO)‐treated BNL CL.2 cells, as well as in CCl 4 , Triton WR‐1339 and corn oil‐treated mice. 2 In G/GO‐treated BNL CL.2 cells, the results showed that GJE glycoprotein has an inhibitory effect on G/GO‐induced cytotoxicity and intracellular reactive oxygen species production. In addition, GJE glycoprotein has an anti‐oxidant effect against the lipid peroxidation process in the Fe 2+ /ascorbic acid system. 3 In CCl 4 (1.0 mL/kg)‐treated mice, pretreatment with GJE glycoprotein (80 mg/kg) blocked lactate dehydrogenase release and the formation of thiobarbituric acid‐reactive substances. In addition, in these mice GJE resulted in increased nitric oxide production and the activation of anti‐oxidant enzymes, accompanied by the inhibition of the cytotoxic‐related signals hepatic cytochrome c , nuclear factor‐κB and activator protein‐1. 4 In both Triton WR‐1339 (400 mg/kg) and corn oil (1.0 g/kg)‐treated mice, pretreatment with GJE glycoprotein (80 mg/kg) lowered the levels of plasma lipoproteins (triglyceride, total cholesterol and low‐density lipoprotein). 5 On the basis of these results, we assume that GJE glycoprotein can ameliorate liver function, because it has hepatoprotective and hypolipidaemic activities.