Premium
BEHAVIOUR OF THE ANTI‐OXIDANT DEFENCE SYSTEM AND HEME OXYGENASE‐1 PROTEIN EXPRESSION IN FRUCTOSE‐HYPERTENSIVE RATS
Author(s) -
Polizio Ariel H,
Gonzales Soledad,
Muñoz Marina C,
Peña Clara,
Tomaro María L
Publication year - 2006
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.2006.04426.x
Subject(s) - oxidative stress , fructose , heme oxygenase , lipid peroxidation , medicine , endocrinology , oxidative phosphorylation , enzyme , kidney , chemistry , heme , biochemistry
SUMMARY1 Addition of fructose to a rat diet for various periods of time leads to hypertension, hyperinsulinaemia and dyslipidaemia and provides a model for testing oxidative stress parameters in the animals. 2 In the present study, oxidative stress generation, the soluble and enzymatic defence system and heme oxygenase‐1 (HO‐1) protein expression were investigated in the heart, liver and kidney of rats fed fructose for a period of 1 or 8 months. 3 Compared with the control group, fructose‐hypertensive rats showed increased in lipid peroxidation only in the heart after both 1 and 8 months of fructose treatment. Changes in the behaviour of the soluble and enzymatic defence system and HO‐1 protein expression were different depending on the organ. Increased or unaltered activities of anti‐oxidant enzymes were found in the liver and kidney, respectively. Induction of HO‐1 prevented the generation of oxidative stress in the liver, where the activity of anti‐oxidant defence enzymes was not reduced. Increased expression of HO‐1 protein was not able to prevent the generation of oxidative stress in the heart, where fructose treatment diminished the activity of anti‐oxidant enzymes. 4 The results of the present study demonstrate that upregulation of HO‐1 may prevent the generation of oxidative stress only when the anti‐oxidant defence system is still operative.