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15‐F 2t ‐ISOPROSTANE and 5‐F 2t ‐ISOPROSTANE ARE NOT TRIGGERS OF MYOCARDIAL PRECONDITIONING
Author(s) -
Arnaud Claire,
Cracowski JeanLuc,
Hakim Ahmed,
Durand Thierry,
Guy Alexandre,
GodinRibuot Diane,
Bessard Germain,
Ribuot Christophe
Publication year - 2005
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.2005.04195.x
Subject(s) - isoprostane , ventricle , isoprostanes , medicine , washout , ethanol , myocardial infarction , chemistry , cardiology , anesthesia , oxidative stress , biochemistry , lipid peroxidation
SUMMARY 1. Myocardial ischaemia–reperfusion in humans is associated with increased formation of 15‐F 2t ‐isoprostane and 5‐F 2t ‐isoprostane (15‐F 2t ‐IsoP and 5‐F 2t ‐IsoP, respectively). Whether this formation is relevant clinically remains controversial. The present study was performed in order to evaluate the ability of the isoprostanes 15‐F 2t ‐IsoP and 5‐F 2t ‐IsoP to reduce myocardial ischaemic injury in rat isolated heart. 2. Rats were divided into six groups. Hearts were excised, perfused retrogradely and pretreated with vehicle (ethanol 5.10 −7 and 2.10 −9 mol/L; n = 6), subjected to ischaemic preconditioning ( n = 8) or pretreated with the isoprostanes 15‐F 2t ‐IsoP (3.10 −10 and 3.10 −7 mol/L; n = 8) or 5‐F 2t ‐IsoP (10 −9 mol/L; n = 8). After a 5 min treatment−5 min washout period, hearts were submitted to 30 min global ischaemia, followed by a 120 min reperfusion period. 3. The infarct‐to‐ventricle zone ratio was significantly reduced in ischaemic preconditioned (20.6 ± 2.6%) compared with vehicle groups (44.5 ± 4.3 and 51.3 ± 2.5% in groups pretreated with 5.10 −7 or 2.10 −9 mol/L ethanol, respectively). Pretreatment with either isoprostane had no cardioprotective effect; the infarct‐to‐ventricle ratios were 43.1 ± 2.2, 49.4 ± 5.9 and 44.5 ± 5.0% for groups treated with 3.10 −10 mol/L 15‐F 2t ‐IsoP, 3.10 −7 mol/L 15‐F 2t ‐IsoP or 10 −9 mol/L 5‐F 2t ‐IsoP, respectively. 4. These data provide evidence that the isoprostanes 15‐F 2t ‐IsoP and 5‐F 2t ‐IsoP are not implicated in early myocardial preconditioning at concentrations similar to those found in the human coronary sinus following coronary angioplasty.