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Effects of 15‐deoxy‐Δ 12,14 ‐prostaglandin J 2 on the expression of Toll‐like receptor 4 and 2 in the murine lung in the presence of lipopolysaccharide
Author(s) -
Inoue Kenichiro,
Takano Hirohisa,
Yanagisawa Rie,
Ichinose Takamichi,
Sadakane Kaori,
Yoshino Shin,
Yamaki Kouya,
Uchiyama Kazuhiko,
Yoshikawa Toshikazu
Publication year - 2005
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.2005.04175.x
Subject(s) - tlr4 , lipopolysaccharide , lung , receptor , toll like receptor , prostaglandin d2 , prostaglandin , chemistry , pharmacology , endocrinology , medicine , innate immune system
Summary 1. Previously, we have demonstrated that 15‐deoxy‐Δ 12,14 ‐prostaglandin J 2 (15d‐PGJ 2 ) enhances acute lung injury induced by lipopolysaccharide (LPS) in mice. The enhancement in acute lung injury by 15d‐PGJ 2 was concomitant with the enhanced expression of several pro‐inflammatory cytokines in the lung. However, other underlying mechanisms of this enhancement remain to be elucidated. The present study investigated the effects of 15d‐PGJ 2 on the expression of Toll‐like receptor (TLR) 4 and 2 in the lung in the absence or presence of LPS. 2. In the present study, ICR mice were divided into four experimental groups that received (intratracheally) vehicle, LPS (125 μg/kg), 15d‐PGJ 2 (1 mg/kg) or 15d‐PGJ 2 + LPS. The mRNA expression of both TLR4 and 2 in the lung was evaluated 4 h after intratracheal administration. 3. 15‐Deoxy‐Δ 12,14 ‐prostaglandin J 2 enhanced the mRNA expression of both TLR4 and 2 in the presence of LPS. 4. These results suggest that the enhancing effects of 15d‐PGJ 2 on LPS‐induced acute lung injury may be explained, at least in part, by its effect on the lung expression of TLR4 and 2.