Premium
Microscopic imaging of extended tissue volumes
Author(s) -
LeGrice Ian,
Sands Greg,
Hooks Darren,
Gerneke Dane,
Smaill Bruce
Publication year - 2004
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.2004.04101.x
Subject(s) - confocal , confocal microscopy , laser scanning , confocal laser scanning microscope , confocal laser scanning microscopy , microscope , laser microscopy , voxel , microscopy , laser , computer science , biomedical engineering , resolution (logic) , computer vision , artificial intelligence , optics , physics , medicine
Summary 1. Detailed information about three‐dimensional structure is key to understanding biological function. 2. Confocal laser microscopy has made it possible to reconstruct three‐dimensional organization with exquisite resolution at cellular and subcellular levels. 3. There have been few attempts to acquire large image volumes using the confocal laser scanning microscope. 4. Previously, we have used manual techniques to construct extended volumes (several mm in extent, at 1.5 µm voxel size) of myocardial tissue. 5. We are now developing equipment and efficient automated methods for acquiring extended morphometric databases using confocal laser scanning microscopy.