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Nitric oxide promotes mitogen‐induced dna synthesis in human dermal fibroblasts through cGMP
Author(s) -
Dhaunsi Gursev S,
Ozand Pinar T
Publication year - 2004
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.2004.03948.x
Subject(s) - sodium nitroprusside , nitric oxide , dna synthesis , wound healing , fibroblast , growth factor , chemistry , fibroblast growth factor , microbiology and biotechnology , dermal fibroblast , dna , endocrinology , pharmacology , medicine , biochemistry , biology , immunology , in vitro , receptor
Summary 1. Nitric oxide (NO) is a free radical with multiple functions in cellular pathophysiology. Nitric oxide has been proven to play an important role in wound healing; however, the mechanisms by which NO may promote wound healing are not clearly understood. We have investigated the effect of NO on growth factor‐induced DNA synthesis in human dermal fibroblasts to suggest interactions between growth factors and NO as a possible mechanism for the role of NO in wound healing. 2. The NO donor sodium nitroprusside (SNP) significantly ( P < 0.001) increased fetal bovine serum‐induced thymidine incorporation into the DNA of human dermal fibroblasts. The maximal comitogenic concentration of SNP (100 µmol/L) was also found to significantly (twofold; P < 0.01) enhance fibroblast growth factor‐ or platelet‐derived growth factor‐induced DNA synthesis. 3. Nitric oxide treatment significantly increased the production of cGMP. 8‐Bromo‐cGMP, a stable structural analogue of cGMP, was found to markedly potentiate ( P < 0.001) the growth factor‐induced DNA synthesis. 4. This study concludes that NO and cGMP promote growth factor‐induced DNA synthesis in dermal fibroblasts, suggesting another possible mechanism by which NO may promote skin wound healing.