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INHIBITION OF K + TRANSPORT IN HUMAN SICKLE CELL ERYTHROCYTES BY OKADAIC ACID AND SODIUM FLUORIDE
Author(s) -
Gharaibeh Nayef Saleh AlRohil,
AlSheyyab† Mahmoud
Publication year - 1997
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1997.tb02700.x
Subject(s) - okadaic acid , cotransporter , sodium fluoride , chemistry , sodium , fluoride , acid phosphatase , phosphatase , biochemistry , inorganic chemistry , enzyme , organic chemistry
SUMMARY 1. The effect of okadaic acid and sodium fluoride on swelling‐and N ‐ethylmaleimide (NEM)‐stimulated KC1 cotransport was examined in blood cells from homozygote sickle cell anaemia patients. 2. Blood was drawn into heparin or EDTA by vein puncture from sickle cell patients previously diagnosed in the haematology clinics of Princess Badee'a Teaching Hospital. A standard method for measuring flux by using radioactive rubidium was used. 3. Okadaic acid strongly inhibited swelling‐stimulated KC1 cotransport if added before swelling. Okadaic acid and sodium fluoride added before NEM inhibited the activation of transport by NEM. Okadaic acid added after NEM did not inhibit transport. 4. The inhibition of the effects of NEM by okadaic acid and sodium fluoride indicates that activation of the flux by NEM requires the action o. phosphatase.