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ASTROCYTE‐CONDITIONED MEDIUM INDUCES BLOOD‐BRAIN BARRIER PROPERTIES IN ENDOTHELIAL CELLS
Author(s) -
Yamagata Kazuo,
Tagami Motoki,
Nara Yasuo,
Mitani Mitsuaki,
Kubota Akiyoshi,
Fujino Hideaki,
Numano Fujio,
Kato§ Taiji,
Yamori Yukio
Publication year - 1997
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1997.tb02117.x
Subject(s) - astrocyte , horseradish peroxidase , blood–brain barrier , endothelial stem cell , chemistry , biophysics , microbiology and biotechnology , vesicular transport protein , vesicle , biochemistry , biology , in vitro , membrane , enzyme , endocrinology , central nervous system
SUMMARY 1. We evaluated the induction of endothelial barrier functions by the type 1 astrocyte‐derived supernatant in culture using horseradish peroxidase (HRP) as a parameter. 2. A gel filtration fraction (molecular weight 16‐20 kDa) of type 1 astrocyte‐derived supernatant reduced plasmalemmal vesicles and induced tight junctions in endothelial cells. 3. In addition, this fraction promoted the uptake of [ 3 H]‐glucosamine by endothelial cells. This cell activity disappeared after heating at 95°C for 10 min and was sensitive to trypsin. 4. These results suggest that a type 1 astrocyte‐derived protein factor(s) promotes proteoglycan synthesis with a resultant increase in charge selectivity in endothelial cells and plays an important role in the induction of blood‐brain barrier functions.