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COMPARISON OF THE EFFECTS OF VASOACTIVE AGENTS ON ADENYLATE CYCLASE ACTIVITY IN ENDOTHELIAL AND SMOOTH MUSCLE CELLS FROM THE SAME SPECIMEN OF FETAL BOVINE AORTA
Author(s) -
Ashikaga Takashi,
Kishi Yukio,
Numano Fujio
Publication year - 1996
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1996.tb03059.x
Subject(s) - cyclase , adenylate kinase , medicine , endocrinology , forskolin , vascular smooth muscle , biology , growth hormone releasing hormone receptor , chemistry , receptor , stimulation , smooth muscle , hormone receptor , cancer , breast cancer
SUMMARY1 Adenylate cyclase activity in vascular endothelial cells (EC) has not been adequately defined. We compared adenylate cyclase activities in EC and smooth muscle cells (SMC) from the same specimen of fetal bovine aorta. 2 The basal adenylate cyclase activities of EC and SMC did not differ significantly (18.9 ± 0.8 and 21.4 ± 1.7 pmol/mg protein per min, n = 10, respectively). 3 The adenylate cyclase of EC responded dramatically to catecholamines, with the ED 50 value for isoproterenol being 0.036 μmol/L, and was also more sensitive to calcitonin gene‐related peptide than that of SMC. 4 The adenylate cyclase of SMC was more sensitive to prostaglandins (with the ED 50 for PGI 2 being 0.024 μmol/L) and glucagon than that of EC, and responded modestly to catecholamines and was almost selectively sensitive to β 1 ‐adrenoceptor agonists. 5 Maximum responses of adenylate cyclase to F − , guanosine 5′‐o‐(3‐thiotriphosphate) and forskolin were greater in SMC than in EC. 6 Based on these findings, it was concluded that EC and SMC differed significantly in adenylate cyclase responsiveness to agonists including hormones, prostaglandins, peptides and substances which may modify the effects of G proteins, although they shared a common developmental origin. Presumably, β‐adrenoceptor agonists in EC and prostaglandins in SMC may appear to play an important role in cellular functions which are mediated by increases in cAMP.