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PCR‐SSCP ANALYSIS OF THE p53 GENE IN TUMOURS OF THE ADRENAL GLAND
Author(s) -
Ballantine DM,
Klemm SA,
Tunny TJ,
Stowasser M.,
Gordon RD
Publication year - 1996
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1996.tb02786.x
Subject(s) - exon , gene , adrenal gland , biology , adenoma , tumor suppressor gene , pathology , cancer research , genetics , endocrinology , medicine , carcinogenesis
SUMMARY 1. Mutations of the p53 tumour suppressor gene are relatively common in the aetiology of a wide spectrum of tumour types, both sporadic and familial. 2. The majority of mutations of the p53 gene are reported to be in the highly conserved region of exons 5–8. 3. Alterations in exons 4, 5 and 7 of the p53 gene in patients with functional adrenal tumours, including aldosterone‐producing adenomas, have recently been described. 4. In the present study PCR‐SSCP was used to examine the exons 4–9 of the p53 gene in paired peripheral blood leucocyte and tumour DNA in a variety of adrenal tumours, including aldosterone‐producing carcinoma and adenoma (both familial and sporadic), phaeochromocytoma and incidentaloma. 5. No evidence was found for mutations in exons 4–9 of the p53 gene in these varieties of adrenal tumours.