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11β‐HYDROXYSTEROID DEHYDROGENASE TYPE I ENZYME IN THE HEARTS OF NORMOTENSIVE AND SPONTANEOUSLY HYPERTENSIVE RATS
Author(s) -
Smith RE,
Krozowski ZS
Publication year - 1996
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1996.tb01750.x
Subject(s) - medicine , endocrinology , corticosterone , 11β hydroxysteroid dehydrogenase type 1 , ventricle , glucocorticoid , enzyme assay , enzyme , dehydrogenase , cortisone , gene expression , metabolism , chemistry , biology , hormone , gene , biochemistry
SUMMARY 1. The enzyme 11β‐hydroxysteroid dehydrogenase (11βHSD) converts glucocorticoids to their inactive 11‐keto metabolites. The ubiquitous expression of the NADP‐dependent isoform (11βHSD1) suggests an important role in modulating glucocorticoid action, but little is known about 11βHSD1 gene expression and enzymatic activity in the rat heart. 2. In the present study rat cardiac 11βHSD1 activity and ontogeny of gene expression have been characterized. The addition of NADP, but not NAD, to heart homogenates resulted in significant increases in the metabolism of both corticosterone and cortisol, with the former substrate displaying far greater metabolism. Both 11βHSD1 gene expression and enzyme activity increased in parallel from low levels at 1 week of age to maximal levels at 8 weeks, with no further change by 16 weeks of age. 3. We also compared theactivity of 11βHSD1 in the hearts of male and female spontaneously hypertensive rats (SHR) with normotensive Wistar‐Kyoto (WKY) controls. Enzyme activity in the pooled atria of female SHR was significantly higher than in male SHR atria (7.6±0.6% conversion of corticosterone vs 4.5±0.5%; P < 0.05). The left ventricles of female WKY rats contained significantly less 11βHSD activity than either male WKY rats or female SHR (8.6±0.8% conversion vs 17±1.4 and 13.6±0.5%, respectively; P < 0.05). In the right ventricle, female WKY rats also had significantly less enzyme activity than either female SHR or male WKY rats (4.9±0.7 vs 10.0±1.7 and 10.2±1.4%; P<0.05). 4. These results clearly show that the rat heart contains significant amounts of the 11βHSD1 enzyme and that this activity is sexually dimorphic. Furthermore, significant differences were observed between a normotensive and hypertensive strain of rat. The relevance of these observations to the aetiology and maintenance of hypertension remains to be explored.