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DISTINCTION OF ENDUTHELIAL CELL GROWTH AND FIBRINOLYTIC ACTIVITY BETWEEN WKY/Izm AND SHRSP/Izm IN VITRO
Author(s) -
Ito Shigeru,
Nara Yasuo,
Yamori Yukio
Publication year - 1995
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1995.tb02913.x
Subject(s) - plasminogen activator , in vitro , medicine , endocrinology , activator (genetics) , chemistry , cell culture , doubling time , microbiology and biotechnology , biology , biochemistry , receptor , genetics
Summary 1. In the present study, endothelial cells (EC) growth and fibrinolytic activity of WKY/Izm and SHRSP/Izm were investigated in vitro . 2. EC were isolated from the thoracic aortas of WKY and SHRSP at the age of 8–9 weeks. Proliferative activities of EC were analysed with doubling time and DNA synthesis. Fibrinolytic activity was determined by tissue type plasminogen activator (tPA) and plasminogen activator inhibitor type 1 (PAI‐1) activities in cultured medium. 3. SHRSP‐EC growth rate was significantly greater than WKY‐EC growth rate in doubling time. In the assay for DNA synthesis, 5‐bromo‐2′‐deoxy uridine incorporation rate in SHRSP‐EC was significantly increased compared with that in WKY‐EC. 4. The tPA activity in cultured medium of WKY‐EC was 2‐fold greater than that of SHRSP‐EC, while PAI‐1 activities were nearly equal in them. 5. These physiological distinctions of EC of SHRSP/Izm from that of WKY/Izm with close genetic background could be contributory genetic factors to hypertension‐related vascular diseases in SHRSP.