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EXPRESSION OF PROTEIN KINASE C GENE IN THE BRAIN AND HEART OF SPONTANEOUSLY HYPERTENSIVE RATS
Author(s) -
Gao Pingjin,
Zhao Guangsheng,
Yuan Xiaoyuan,
Zhao Hanfang,
Chen Feng,
Chen Shishu
Publication year - 1995
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1995.tb02884.x
Subject(s) - protein kinase c , protein kinase a , medicine , gene , gene expression , kinase , chemistry , endocrinology , biochemistry
Summary 1. The aim of this study was to investigate protein kinase C (PKC) gene expression in spontaneously hypertensive rats (SHR). 2. Using the PKC oligodeoxyribonucleotide probes (γ, ε), we detected PKC isoforms gene expression in the heart and brain of 4 and 20 week old SHR with those of age‐matched Wistar‐Kyoto (WKY) rats by northern blot analysis. 3. In the cerebral cortex, there were significantly increased levels of expression of the Ca 2+ ‐dependent isoform PKC‐γ in 4 and 20 weeks SHR compared with that of WKY, while Ca 2+ ‐independent isoform PKC‐ε did not differ between SHR and WKY. 4. In ventricular myocytes, there was a significant expression of the Ca 2+ ‐independent isoform PKC‐ε in 4 and 20 week old SHR compared with that of WKY, while Ca 2+ ‐dependent isoform PKC‐γ could not be detected in the same extracts of SHR or WKY. 5. We conclude that both of the Ca 2+ ‐dependent and Ca 2+ ‐independent PKC could be involved in the pathogenesis of SHR. Ca 2+ ‐dependent PKC‐γ may be mainly involved in the modulation of blood pressure in the level of the central nervous system, while Ca 2+ ‐independent PKC‐ε Could be related to the genetic myocardial hypertrophy.