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EFFECT OF THROMBIN AND PDGF ON ENDOTHELIN PRODUCTION IN CULTURED MESANGIAL CELLS DERIVED FROM SPONTANEOUSLY HYPERTENSIVE RATS
Author(s) -
Ikeda Miwako,
Kohno Masakazu,
Horio Takeshi,
Yasunari Kenichi,
Yokokawa Koji,
Kano Hiroaki,
Minami Mieko,
Hanehira Takao,
Fukui Toshiki,
Takeda Tadanao
Publication year - 1995
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1995.tb02879.x
Subject(s) - thrombin , endothelin 1 , platelet derived growth factor receptor , medicine , endocrinology , mesangial cell , endothelin receptor , endothelins , chemistry , platelet , kidney , growth factor , receptor
Summary 1. Basal endothelin‐1 (ET‐1) production in mesangial cells of spontaneously hypertensive rats (SHR) was not different from that of Wistar‐Kyoto (WKY) rats, although a trend toward increased ET‐1 production was observed in these cells of SHR. 2. Thrombin and platelet‐derived growth factor (PDGF) stimulated ET‐1 production in a concentration‐dependent manner in these cells of both rat strains, but thrombin‐ and PDGF‐induced stimulation of ET‐1 production were clearly greater in cells of SHR than WKY rats. 3. The protein kinase C (PKC)‐activating phorbol ester, phorbol myristate acetate, stimulated ET‐1 production in cells of both rat strains, but this stimulation was significantly greater in cells of SHR than in cells of WKY rats. 4. An inactive enantiomer of phorbol ester, 4a‐PDD, had no effect on the ET‐1 production in these cells of both rat strains. 5. Neither thrombin nor PDGF stimulated ET‐1 production in PKC‐depleted cells of both rat strains.