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AUGMENTED EXPRESSION OF THE ENDOTHELIN‐A RECEPTOR GENE IN CULTURED MESANGIAL CELLS FROM STROKE‐PRONE SPONTANEOUSLY HYPERTENSIVE RATS
Author(s) -
Hiraoka Junko,
Arai Hiroshi,
Yoshimasa Takaaki,
Takaya Kazuhiko,
Miyamoto Yoshihiro,
Yamashita Jun,
Suga Shinichi,
Ogawa Yoshihiro,
Shirakami Gotaro,
Itoh Hiroshi,
Yamori Yukio,
Nakao Kazuwa
Publication year - 1995
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1995.tb02876.x
Subject(s) - medicine , endocrinology , endothelin receptor , endothelin 1 , northern blot , spontaneously hypertensive rat , messenger rna , gene expression , receptor , mesangial cell , gene , chemistry , microbiology and biotechnology , biology , blood pressure , kidney , biochemistry
Summary 1. To elucidate the role of endothelin (ET) receptor in hypertension, we studied the expression of the ET‐A receptor (ET‐ AR) gene and the ET‐B receptor (ET‐BR) gene in cultured mesangial cells isolated from stroke‐prone spontaneously hypertensive rats (SHRSP) and Wistar‐Kyoto (WKY) rats. 2. The mesangial cells from both SHRSP and WKY expressed ET‐AR predominantly. The level of the ET‐AR mRNA in mesangial cells from SHRSP was 5‐fold higher than that in the cells from WKY. The ET‐BR mRNA in the mesangial cells from both strains was hardly detectable by northern blot analysis. 3. These results demonstrate that the expression of the ET‐AR gene was markedly augmented in mesangial cells from SHRSP.