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ALTERNATIVE SPLICING OF NATRIURETIC PEPTIDE A AND B RECEPTOR TRANSCRIPTS IN THE RAT BRAIN
Author(s) -
Francoeur F.,
Gossard F.,
Hamet P.,
Tremblay J.
Publication year - 1995
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1995.tb02869.x
Subject(s) - npr2 , alternative splicing , rna splicing , receptor , npr1 , natriuretic peptide , microbiology and biotechnology , biology , neuroscience , endocrinology , medicine , gene , genetics , heart failure , rna , messenger rna
Summary 1. In the present study we searched for variants of alternative splicing of guanylyl cyclase A and B mRNA in rats in vivo . 2. Guanylyl cyclase A2 and guanylyl cyclase B2 isoforms of guanylyl cyclase produced by alternative splicing leading to the deletion of exon 9 of both transcripts were quantified in several rat organs. 3. Only one alternative splicing was found in the regulatory domain, encoded by exons 8–15. 4. Quantification of the guanylyl cyclase B2 isoform in different rat organs and in cultured aortic smooth muscle cells showed that this alternative splicing was tissue‐specific and occurred predominantly in the central nervous system where the alternatively spliced variant represented more than 50% of the guanylyl cyclase B mRNA. 5. The same alternative splicing existed for guanylyl cyclase A mRNA but at very low levels in the organs studied. 6. Alternative splicing of guanylyl cyclase B exon 9 in the brain may play an important role in signal transduction, since the expressed protein possesses a constitutionally active guanylyl cyclase acting independently of C‐type natriuretic peptide regulation.