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DISTINCT EXPRESSION OF TRANSFORMING GROWTH FACTOR‐β RECEPTOR SUBTYPES ON VASCULAR SMOOTH MUSCLE CELLS FROM SPONTANEOUSLY HYPERTENSIVE RATS AND WISTAR‐KYOTO RATS
Author(s) -
Fukuda Noboru,
Kubo Atsushi,
Izumi Yoichi,
Soma Masayoshi,
Kanmatsuse Katsuo
Publication year - 1995
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1995.tb02847.x
Subject(s) - vascular smooth muscle , medicine , receptor , endocrinology , transforming growth factor , cell , biology , receptor expression , smooth muscle , chemistry , biochemistry
Summary 1. We investigated the characteristics and expression of transforming growth factor‐β (TGFB) receptor subtypes on vascular smooth muscle cells (VSMC) from spontaneously hypertensive rats (SHR) and normotensive Wistar‐Kyoto (WKY) rats. 2. TGF β binding assays revealed that VSMC from SHR showed higher number and affinity of TGF β receptors than cells from WKY rats. 3. The affinity labelling with [ 125 I]‐TGFβ 1 revealed the presence of TGFβ receptor subtypes with molecular sizes of 280, 85, 70 and 60kDa on VSMC from both strains at high cell density. The abundance of the 85 kDa receptor subtype was greater in VSMC from SHR than cells from WKY rats. The 85 kDa receptor subtype was not detected on VSMC from both strains at low cell density. 4. These results suggest distinct expression of TGFP receptor subtypes on VSMC from SHR and WKY rats, which may account for the exaggerated proliferative response of VSMC from SHR to TGFβ.