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ANALYSIS OF THE RENIN GENE IN PATIENTS WITH ALDOSTERONE‐PRODUCING ADENOMAS BY POLYMERASE CHAIN REACTION‐SINGLE STRANDED CONFORMATIONAL POLYMORPHISMS AND LONG POLYMERASE CHAIN REACTION
Author(s) -
Ballantine Donna M.,
Klemm Shelley A.,
Tunny Terry J.,
Stowasser Michael,
Gordon Richard D.
Publication year - 1995
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1995.tb02052.x
Subject(s) - renin–angiotensin system , exon , aldosterone , polymerase chain reaction , gene , medicine , angiotensin ii , endocrinology , polymorphism (computer science) , plasma renin activity , microbiology and biotechnology , biology , chemistry , genotype , genetics , blood pressure
SUMMARY 1. Angiotensin‐responsive aldosterone‐producing adenomas (AII‐R‐APA) have increased expression of renin mRNA compared with angiotensin‐unresponsive aldosterone‐producing adenomas (AII‐U‐APA) or normal adrenals. 2. Further, significant associations between the Bgl I, Taq I and Hinf I RFLP and aldosterone responsiveness to the renin‐angiotensin system of the two subgroups of patients have been reported. 3. Using the polymerase chain reaction based technique single stranded conformational polymorphism, we detected no alterations in exon 1 of the renin gene in peripheral blood leucocyte DNA from normal AII‐U‐APA and AII‐R‐APA subjects. 4. Using long‐PCR, we amplified a fragment of the renin gene consisting of a region covering 500 bp upstream of exon 1, exon 1 and intron A. No gross changes in this area of the renin gene were found in the three groups of subjects studied. However this does not exclude small alterations in this area.