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GENE EXPRESSION OF ENDOTHELIN RECEPTORS IN AORTIC CELLS FROM CYCLOSPORINE‐INDUCED HYPERTENSIVE RATS
Author(s) -
Iwai Junko,
Kanayama Yoshiharu,
Negoro Nobuo,
Okamura Mikio,
Takeda Tadanao
Publication year - 1995
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1995.tb02030.x
Subject(s) - endothelin receptor , receptor , messenger rna , endocrinology , medicine , endothelium , endothelin 1 , biology , reverse transcription polymerase chain reaction , vascular smooth muscle , chemistry , smooth muscle , gene , biochemistry
SUMMARY 1. We examined preproendothelin‐1, ET A and ETb receptor mRNA levels in aortic endothelial and smooth muscle cells from cyclosporine (CyA)‐induced hypertensive rats using the reverse transcription polymerase chain reaction method. 2. Aortic endothelial preproendothelin‐1 mRNA expression was about 1.5‐fold higher, while that of ET B receptor mRNA was markedly decreased in CyA‐treated rats compared with those in controls. 3. The expression of ET A receptor mRNA in smooth muscle cells from CyA‐induced hypertensive rats was increased about two‐fold over that in cells from control animals. 4. Thus, increased endothelial preproendothelin‐1, and ETa receptor mRNA levels in smooth muscle cells, which are concomitant with the decrease in ET B receptor mRNA levels in endothelium, may contribute to CyA‐induced hypertension in rats.