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USE OF DIFFERENTIATED CHOLINERGIC AND SECOND MESSENGER ENDPOINTS TO EVALUATE CHOLINESTERASE INHIBITORS
Author(s) -
Blusztajn J. K.,
Davis Ross O.
Publication year - 1995
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1995.tb02020.x
Subject(s) - paraoxon , cholinergic , cholinesterase , acetylcholine , intracellular , chemistry , second messenger system , acetylcholinesterase , pharmacology , endocrinology , medicine , enzyme , biochemistry , biology
SUMMARY 1. The effects of organophosphorus compounds on the cholinergic properties of a mouse septal neuronal cell line, SN56, were investigated. Treatment of the cells with 10 μmol/L paraoxon or 10 μmol/L diisopropylphosphorofluoridate (DFP) resulted, respectively, in rapid 27‐ and 11‐fold increases of intracellular acetylcholine content. 2. Time‐course studies showed that paraoxon maintained its efficacy whereas the effects of DFP decayed quickly, possibly due to rapid hydrolysis and inactivation of DFP in the growth medium.