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APOPTOTIC AN IN AN IN VITRO MODEL OF NEURONAL OXIDATIVE STRESS
Author(s) -
Ratan Rajiv R.,
Baraban Jay M.
Publication year - 1995
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1995.tb02006.x
Subject(s) - oxidative stress , apoptosis , programmed cell death , microbiology and biotechnology , in vitro , glutathione , chemistry , oxidative phosphorylation , biology , biochemistry , enzyme
SUMMARY 1. Oxidative stress is believed to be an important mediator of neuronal cell death but the precise mechanism by which this accurs is unknown. 2. We have developed an in vitro model of neuronal oxidative stress to study the pathways by which free radicals kill neurones. 3. We have shown that oxidative stress, cystine deprivation and glutathione depletion results in cell death with the morphological and biochemical features of apoptosis. 4. Neuronal apoptosis induced by oxidative stress can be inhibited by macromolecular synthesis inhibitors. 5. This in vitro model will be a valuable tool for defining the molecular targets of toxic free radicals in neurones and, in turn, in designing rational new therapies for free radical mediated diseases.