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PRACTICAL APPLICATIONS OF NEURONAL TISSUE CULTURE IN IN VITRO TOXICOLOGY
Author(s) -
Sawyer Thomas W.
Publication year - 1995
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1995.tb02000.x
Subject(s) - organophosphate , in vitro , nerve agent , neutral red , cytotoxicity , toxicology , primary culture , tissue culture , in vitro toxicology , cell culture , biology , mtt assay , pharmacology , chemistry , pesticide , biochemistry , acetylcholinesterase , genetics , agronomy , enzyme
SUMMARY 1. Primary chick embryo forebrain neurones are relatively easy to culture and are quite resilient to treatment manipulation. These characteristics have allowed application in a surprisingly diverse number of areas. 2. These cultures have been used to investigate the anti‐cholinesterase potencies of many organophosphate (OP) nerve agents and insecticides. 3. These cultures have been used to quantitate levels of OP in ‘spiked’ unknowns and in OP‐contaminated soil samples. 4. These cells have also been used to test a variety of compounds using the MTT and neutral red cytotoxicity assays.