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EFFECTS OF SELECTED ORGANIC SOLVENTS ON THE ASTROCYTE MEMBRANE ATPase IN VITRO
Author(s) -
Vaalavirta Leila,
Tähti Hanna
Publication year - 1995
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1995.tb01999.x
Subject(s) - chemistry , astrocyte , toluene , ethylbenzene , atpase , benzene , membrane , in vitro , styrene , xylene , cyclohexane , biochemistry , enzyme , organic chemistry , biology , central nervous system , polymer , neuroscience , copolymer
SUMMARY 1. The present study deals with astrocyte cultures as a model for studying the membrane‐mediated central nervous system‐depressing effect of organic solvents. 2. The primary astrocyte cultures were prepared from neonatal rat cerebella. The cells were cultured in modified essential medium. The astrocyte membranes isolated from the cultures were exposed to solvents in incubation mixture at different dose levels (3, 6 and 9 mmol/L) for 1h. The physiologically important integral proteins Na + , K + ‐ATPase and Mg 2+ ‐ATPase were studied. 3. The aromatic hydrocarbons (benzene, toluene, styrene, xylene and ethylbenzene) inhibited the ATPase activities according to their lipid solubilities. n ‐Hexane and cyclohexane clearly had less effect than aromatic hydrocarbons, despite their greater lipid solubilities. 4. Astrocytes were shown to be sensitive targets to the effects of organic solvents, measured as the inhibition of the integral enzymes Na + , K + ‐ATPase and Mg 2+ ‐ATPase.