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INVOLVEMENT OF TRANSFORMING GROWTH FACTOR‐β 1 FOR PLATELETS‐INDUCED STIMULATION OF ENDOTHELIN‐1 PRODUCTION
Author(s) -
Matsumura Yasuo,
Murata Satoshi,
Takada Kimi,
Takaoka Masanori,
Morimoto Shiro
Publication year - 1994
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1994.tb02661.x
Subject(s) - platelet , thrombin , secretion , stimulation , chemistry , transforming growth factor , endothelin 1 , medicine , endocrinology , growth factor , platelet activation , microbiology and biotechnology , biology , biochemistry , receptor
SUMMARY 1. Possible mechanisms by which platelets stimulate the production of endothelin‐1 (ET‐1) in vascular endothelial cells (EC) were investigated. 2. A supernatant of platelets stimulated the expression of prepro ET‐1 mRNA, followed by an increased secretion of ET‐1 from cultured EC. These responses were markedly enhanced by pretreatment of the platelets with thrombin, at a concentration which did not influence the ET‐1 production in EC. A platelet suspension, separated from cultured EC by a permeable nylon membrane, also markedly stimulated the ET‐1 secretion from EC. 3. Endothelin‐1 production enhanced by the platelet supernatant, with or without thrombin pretreatment, correlated with the active transforming growth factor‐β 1 (TGF‐β 1 ) concentrations in the supernatant. The supernatant‐induced increase in ET‐1 secretion from the EC was markedly suppressed by TGF‐β 1 neutralizing antibody. 4. We tentatively conclude that platelets stimulate the endothelial ET‐1 production by releasing a bioactive diffusible substance, mainly TGF‐β 1 .