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ANTISENSE OLIGONUCLEOTIDES: A NEW TOOL IN NEUROSCIENCE
Author(s) -
Pilowsky Paul M.,
Suzuki Satoshi,
Minson Jane B.
Publication year - 1994
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1994.tb02655.x
Subject(s) - oligonucleotide , sense (electronics) , computational biology , gene , rna , antisense rna , gene expression , messenger rna , antisense therapy , dna , biology , chemistry , genetics , locked nucleic acid
SUMMARY 1. Recent studies have shown that gene expression can be selectively attenuated by administration of short sequences of nucleotides (oligonucleotides) that are complementary to a portion of messenger RNA coding for a particular gene product. 2. This technique is known as ANTISENSE, because the oligonucleotides are complementary to the mRNA which has the same sequence as the SENSE strand of DNA. 3. In the present review we focus, after a brief discussion of gene expression and mechanisms of action of ANTISENSE, on the methodological aspects of ANTISENSE experiments in neuroscience. In particular, we address the advantages, disadvantages and controls for the ANTISENSE technique, as well as the choice, design, mode of delivery, dose and storage of ANTISENSE oligonucleotides.