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BLOOD VOLUME MEASUREMENTS IN THE NEONATAL LAMB: VALIDATION OF A METHOD USING [ 51 Cr]‐LABELLED RED CELLS
Author(s) -
Moritz Karen M.,
Potocnik Simon J.,
Tangalakis Kathy,
Wintour E. Marelyn
Publication year - 1994
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1994.tb02557.x
Subject(s) - volume (thermodynamics) , blood volume , nuclear medicine , radiochemistry , chemistry , physics , medicine , cardiology , thermodynamics
SUMMARY 1. The reproducibility of blood volume measurements, using [ 51 Cr]‐labelled red blood cells (RBC) was tested in three lambs, 79–89 days of age, and the accuracy was tested in eight lambs, 83± 0.4 days of age, in which blood volume was measured simultaneously by [ 51 Cr]‐RBC and [ 125 I]‐gamma globulin plus haematocrit (hct). 2. The blood volumes (mean ± s.e.) of the three lambs, on four occasions, were 64.5± 2.5, 68.8 ± 2.9 and 63.9 ± 3.7 mL/kg, respectively, and the coefficients of variation were 7.8, 8.6 and 11.6%, respectively. 3. The mean blood volume of eight lambs was 60.9 ± 3.3 mL/kg by [ 51 Cr]‐RBC, and 60.8 ± 2.4 mL/ kg from plasma volume and hct. These were not statistically different. 4. Arterial hct was 31.2 ± 0.5%, which was not statistically different from the whole body hct of 31.2 ±2.6%. 5. It is concluded that [ 51 Cr]‐RBC can be used to measure accurate and reproducible blood volumes in lambs.