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SPONTANEOUS ENDOTHELIN PRODUCTION BY CIRCULATING MONONUCLEAR CELLS FROM PATIENTS WITH CHRONIC HEART FAILURE BUT NOT FROM NORMAL SUBJECTS
Author(s) -
Krum Henry,
Itescu Silviu
Publication year - 1994
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1994.tb02518.x
Subject(s) - heart failure , peripheral blood mononuclear cell , medicine , endothelin 1 , endocrinology , stimulation , monocyte , chemistry , receptor , in vitro , biochemistry
SUMMARY 1. Plasma endothelin‐1 (ET‐1) levels are increased in chronic heart failure (CHF). Because chronic immunologic activation occurs in CHF and mononuclear cells (MNC) are capable of ET‐1 production, the possible contribution of circulating MNC to the increased plasma ET‐1 levels in CHF patients was investigated. 2. Unseparated MNC (10 7 /mL) from eight CHF patients spontaneously produced ET‐1 (1.54 ± 0.37 pg/100 mL). After separation of MNC into monocytes (10 6 /mL) and lymphocytes (10 7 /mL) at ratios approximating those in the circulation, ET‐1 was spontaneously produced by CHF lymphocytes (0.81 ± 0.26 pg/100 mL), and after macrophage colony stimulating factor (MCSF) stimulation by CHF monocytes (0.56 ± 0.08 pg/100 mL). In contrast, there was neither spontaneous nor MCSF‐induced production of ET‐1 in six normal subjects. 3. It was concluded that heightened ET‐1 production by MNC may contribute to increased plasma levels in CHF patients. Because unseparated MNC produced greater ET‐1 than lymphocytes and monocytes separately, contact between these MNC subtypes may be necessary for maximal ET‐1 production.