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EFFECT OF TRANSFORMING GROWTH FACTOR‐β1 ON PLATELET‐DERIVED GROWTH FACTOR RECEPTOR BINDING AND GENE EXPRESSION IN VASCULAR SMOOTH MUSCLE CELLS FROM SHR AND WKY RATS
Author(s) -
Agrotis Alex,
Saltis John,
Bobik Alex
Publication year - 1994
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1994.tb02484.x
Subject(s) - platelet derived growth factor receptor , endocrinology , growth factor , medicine , transforming growth factor , platelet derived growth factor , vascular smooth muscle , dna synthesis , receptor , intracellular , messenger rna , biology , chemistry , gene , microbiology and biotechnology , dna , smooth muscle , biochemistry
SUMMARY 1. This study examined the effects of transforming growth factor‐βl (TGF‐β1) on platelet‐derived growth factor‐BB (PDGF‐BB)‐stimulated DNA synthesis, [ 125 I]‐PDGF‐BB receptor binding and PDGF‐β receptor mRNA expression in vascular smooth muscle cells (VSMC) isolated from Wistar‐Kyoto (WKY) rats and spontaneously hypertensive rats (SHR). 2. TGF‐β1 inhibited by 40% DNA synthesis stimulated by PDGF‐BB in VSMC from WKY rats but potentiated by 20% growth factor‐stimulated DNA synthesis in VSMC from the SHR. 3. Since the difference in effect of TGF‐β1 could not be attributed to differential regulation of [ 125 I]‐PDGF‐BB binding activity and PDGF‐β receptor mRNA expression, it is suggested that alterations in intracellular signalling pathways may account for the differential effects of TGF‐β1 on PDGF‐BB‐stimulated DNA synthesis in VSMC from SHR and WKY rats.