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HIGH FISH OIL DIET INCREASES OXIDATIVE STRESS POTENTIAL IN MAMMARY GLAND OF SPONTANEOUSLY HYPERTENSIVE RATS
Author(s) -
Mehta R. S.,
Gunnett C. A.,
Harris S. R.,
Bunce O. R.,
Hartle D. K.
Publication year - 1994
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1994.tb02459.x
Subject(s) - oxidative stress , fish oil , fish <actinopterygii> , mammary gland , endocrinology , medicine , oxidative phosphorylation , chemistry , physiology , biology , biochemistry , fishery , cancer , breast cancer
SUMMARY 1. The purpose of this study was to determine whether high ω‐3 (19% menhaden oil, 1% corn oil) or high ω‐6 (20% corn oil) fatty acid diets would decrease expression of hypertension in the female spontaneously hypertensive rat (SHR), promote tumourigenesis in the rat 7,12‐dimethyl‐benz[a]anthracene (DMBA) model of mammary cancer or increase the susceptibility of the mammary gland to lipid peroxidation. A group of rats on a 5% corn oil diet served as the low fat control group. 2. We found that the high ω‐3 and high ω‐6 fatty acid diets did not significantly decrease mean arterial pressure. Marked differences occurred between the effects of ω‐3 and ω‐6 high fatty acid diets on baseline oxidation, auto‐oxidation and iron‐ascorbate catalyzed oxidation. The ω‐3 diet showed 675% increase in basal oxidation, a 2624% increase in auto‐oxidation and a 4244% increase in iron‐ascorbate catalyzed oxidation compared to the ω ‐6 diet in mammary tissue homogenates. Although all rats were given 5 mg DMBA (i.g.), no mammary tumours were observed in any of the dietary groups. 3. We conclude that: (i) high polyunsaturated fatty acid diets do not decrease blood pressure in the female SHR; (ii) high fish oil diet markedly increases oxidative potential in the mammary gland; and (iii) the female SHR is resistant to DMBA‐induced tumourigenesis.