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INTRALYMPHOCYTIC FREE CALCIUM AND MAGNESIUM IN STROKE‐PRONE SPONTANEOUSLY HYPERTENSIVE RATS AND EFFECTS OF BLOOD PRESSURE AND VARIOUS ANTIHYPERTENSIVE AGENTS
Author(s) -
Adachi Minoru,
Nara Yasuo,
Mano Masayuki,
Ikeda Katumi,
Horie Ryoichi,
Yamori Yukio
Publication year - 1993
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1993.tb01745.x
Subject(s) - magnesium , blood pressure , calcium , stroke (engine) , medicine , pharmacology , spontaneously hypertensive rat , anesthesia , chemistry , mechanical engineering , organic chemistry , engineering
SUMMARY 1. Free Ca 2+ ([Ca 2+ ]i) and Mg 2+ ([Mg 2+ ]i) were measured in peripheral lymphocytes from stroke‐prone spontaneously hypertensive rats (SHRSP) and normotensive Wistar‐Kyoto rats (WKY) at the age of 5, 7 and 17 weeks, from various antihypertensive agents‐treated SHRSP, and from secondary hypertensive WKY. 2. At the age of 5 weeks, no difference was observed in systolic blood pressure (SBP), or lymphocyte [Ca 2+ ]i and [Mg 2+ ]i between SHRSP and WKY. At the age of 7 or 17 weeks, SBP and [Ca 2+ ]i of SHRSP were significantly higher than in WKY, and at the age of 17 weeks, [Mg 2+ ]i of SHRSP was significantly lower than in WKY. Further, [Ca 2+ ]i or [Mg 2+ ]i was positively or negatively correlated to SBP, and [Mg 2+ ]i was negatively correlated to [Ca 2+ ]i. 3. SBP of SHRSP fell significantly after antihypertensive treatment with calcium antagonist, angiotensin‐converting enzyme (ACE) inhibitor or hydralazine for 40 days. [Ca 2+ ]i was significantly lower in calcium antagonist and hydralazine groups, and tended to be low in ACE inhibitor group. These four groups showed no difference in [Mg 2+ ]i. 4. After 40‐day administration of N G ‐nitro‐l‐arginine (l‐NNA), WKY developed severe hypertension, but there were no significant differences in lymphocyte [Ca 2+ ]i and [Mg 2+ ]i between the l‐NNA treated and non‐treated groups. 5. These results suggested that increased lymphocyte [Ca 2+ ]i and decreased [Mg 2+ ]i observed in SHRSP are not only secondary to hypertension but possibly related to a basic genetic abnormality of divalent cation handling.