Premium
ANALYSIS OF SECRETION AND EXPRESSION OF PLATELET‐DERIVED GROWTH FACTOR IN CULTURED ENDOTHELIAL CELLS FROM STROKE‐PRONE SPONTANEOUSLY HYPERTENSIVE RAT
Author(s) -
Iihara Koji,
Sasahara Masakiyo,
Saeki Yukikazu,
Hashimoto Nobuo,
Kikuchi Haruhiko,
Hazama Fumitada
Publication year - 1993
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1993.tb01734.x
Subject(s) - endocrinology , medicine , growth factor , platelet derived growth factor , platelet derived growth factor receptor , endothelial stem cell , secretion , cell growth , platelet , cell , vascular endothelial growth factor b , vascular endothelial growth factor , biology , chemistry , microbiology and biotechnology , vascular endothelial growth factor a , vegf receptors , in vitro , biochemistry , receptor
SUMMARY 1. We characterized the endothelial cell‐derived growth factors of SHRSP and Wistar Kyoto rats (WKY), respectively and found that platelet‐derived growth factor (PDGF)‐B chain related growth factor constituted a major portion of the mitogenic activity of the conditioned media of endothelial cells from both animals. There were no remarkable qualitative differences between the endothelial cell‐derived growth factors of SHRSP and WKY. 2. Northern analysis revealed that the expression of PDGF‐B chain was 2–4‐fold enhanced in cultured aortic endothelial cells of SHRSP. This enhanced expression of PDGF‐B chain, which may be induced under chronic hypertensive conditions, is suggested to contribute to the increase in endothelial cell‐derived growth factors reported in this animal.