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AGE‐DEPENDENT ALTERATIONS IN VASCULAR SMOOTH MUSCLE CELL RESPONSIVENESS TO PLATELET‐DERIVED GROWTH FACTOR IN GENETIC HYPERTENSION
Author(s) -
Saltis John,
Agrotis Alex,
Bobik Alex
Publication year - 1993
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1993.tb01695.x
Subject(s) - vascular smooth muscle , medicine , endocrinology , growth factor , platelet derived growth factor , platelet derived growth factor receptor , downregulation and upregulation , stimulation , platelet , spontaneously hypertensive rat , smooth muscle , biology , blood pressure , receptor , biochemistry , gene
SUMMARY 1. This study examined and compared the growth characteristics of vascular smooth muscle cells (VSMC) isolated from 4 and 12 week old spontaneously hypertensive rats (SHR) and Wistar‐Kyoto (WKY) rats following stimulation with platelet‐derived growth factor‐BB (PDGF‐BB). 2. Vascular smooth muscle cells from 4 week old SHR proliferated at a slower rate than VSMC isolated from 12 week old SHR (1.08±0.06/day vs 1.89±0.13/day respectively, P < 0.05). In contrast, there was no difference in the proliferation of VSMC from 4 and 12 week WKY rats (0.62±0.06/day vs 0.82±0.13/day, respectively, P >0.05). 3. The cell density at which VSMC from 4 and 12 week old SHR become refractory to the mitogenic effects of PDGF‐BB was similar and approximately two‐fold greater than VSMC from age‐matched WKY rats. 4. This study concludes that there is an age‐dependent, differential and specific upregulation of growth rate mechanisms in VSMC from SHR which enhance proliferation in response to PDGF‐BB.