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DETECTION OF RENIN mRNA IN ALDOSTERONE‐PRODUCING ADENOMAS BY POLYMERASE CHAIN REACTION
Author(s) -
Klemm Shelley,
Pinet Florence,
RioualCaroff Nathalie,
Tunny Terry,
Corvol Pierre,
Gordon Richard
Publication year - 1993
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1993.tb01689.x
Subject(s) - renin–angiotensin system , aldosterone , messenger rna , endocrinology , medicine , complementary dna , aldosterone synthase , rna , angiotensin ii , reverse transcriptase , in vivo , chemistry , biology , gene , biochemistry , blood pressure , genetics
SUMMARY 1. Ribonucleic acid (RNA) was extracted from two normal human adrenal cortices and from five aldosterone‐producing adenomas (APA). 2. The five APA could be categorized, on the basis of in vivo aldosterone responsiveness to angiotensin infusion and upright posture, into two APA responsive to both stimuli, two responsive only to angiotensin infusion, and one unresponsive to either stimulus. 3. RNA was reverse transcribed and coamplified by polymerase chain reaction (PCR) with an internal standard of renin complementary DNA (cDNA) containing a 60 base pair insertion. Renin mRNA in the APA was compared with normal adrenals. 4. Renin mRNA was greater than normal in the two APA responsive to both stimuli and less than, or similar to normal, in the two APA responsive only to angiotensin infusion. Renin mRNA was also less than, or similar to normal, in the APA unresponsive to either stimulus. 5. These findings support a possible role for adrenal renin in the development and biochemical behaviour of angiotensin‐responsive APA.