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SPECIES DIFFERENCES IN BINDING OF SUBMANDIBULAR NUCLEAR PROTEINS TO RENIN PROMOTER DNA
Author(s) -
Loudon John A.,
Fukamizu Akiyoshi,
Murakami Kazuo,
Morris Brian J.
Publication year - 1993
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1993.tb01684.x
Subject(s) - dna , renin–angiotensin system , submandibular gland , chemistry , dna binding protein , biochemistry , microbiology and biotechnology , biology , endocrinology , transcription factor , gene , blood pressure
SUMMARY 1. Renin is highly expressed in submandibular gland (SMG) of mouse, which has two genes, Ren‐1 d and Ren‐2 d , but not at all in rat SMG. Differences in nuclear protein binding to renin promoter DNA were, therefore, explored. 2. Rat ‐169 to +23 renin DNA formed complexes with both mouse and rat extract, whereas a corresponding fragment of mouse Ren‐Id DNA (‐121 to +4) bound with rat extract, but much less so with mouse extract. Rat extract bound a ‐704 to ‐450 fragment of the Ren‐1 d promoter. For Ren‐2 d ‐578 to ‐383 and ‐786 to ‐718 DNA bound with mouse extract and ‐383 to +11 and ‐664 to ‐ 578 DNA bound with rat extract. 3. The results support a role for differences in presence or binding of species‐specific trans‐acting factors in the differential regulation of the renin gene in SMG of mouse and rat. Strong binding near the rat RNA polymerase II binding site could repress transcription in rat SMG, and binding peculiar to the Ret‐2 d B2 element might contribute to high expression in mouse SMG.